Biotechnology: Principles and Processes

Which of the following statements are not true regarding restriction endonucleases? Choose the answer from the options given below :

1. C and D only
2. A and E only
3. D and E only
4. A and B only

Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction :

1. Denaturation Extension Annealing
2. Denaturation Annealing Extension
3. Annealing Denaturation Extension
4. Extension Annealing Denaturation

Which of the following statements are correct with respect to DNA separation, isolation and visualization? Choose the correct answer from the options given below :

1. A and B only
2. B and D only
3. A and D only
4. B and C only

Insertion of a foreign DNA at BamHI site in an E. coli cloning vector pBR322 results in the loss of antibiotic resistance towards :

1. Ampicillin and tetracycline
2. Ampicillin
3. Tetracycline
4. Gentamycin

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). In the light of the above statements, choose the most appropriate answer from the options given below:

1. A is not correct but R is correct
2. Both A and R are correct and R is the correct explanation of A
3. Both A and R are correct but R is not the correct explanation of A
4. A is correct but R is not correct

Given below are two statements: In the light of the above statements, choose the most appropriate answer from the options given below:

1. Statement I is incorrect but Statement II is correct
2. Both Statement I and Statement II are correct
3. Both Statement I and Statement II are incorrect
4. Statement I is correct but Statement II is incorrect

During PCR, primers bind to the DNA strands in the step.

1. ligation
2. denaturation
3. extension
4. annealing

[figure] In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?

1. White color colonies will be selected.
2. Blue color colonies grown on ampicillin plates can be selected.
3. Using ampicillin & tetracycline containing medium plate.
4. Blue color colonies will be selected.

Polymerase chain reaction (PCR) amplifies DNA following the equation.

1. 2n+1
2. 2 N2
3. N2
4. 2n

Identify the part of a bio-reactor which is used as a foam breaker from the given figure. [figure]

1. D
2. C
3. A
4. B

The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate. Given below are two statements about this method: In the light of the above statements, choose the most appropriate answer from the options given below:

1. Statement I is correct but Statement II is incorrect
2. Statement I is incorrect but Statement II is correct
3. Both Statement I and Statement II are correct
4. Both Statement I and Statement II are incorrect

Which of the following enzyme(s) are NOT essential for gene cloning? Choose the correct answer from the options given below:

1. D and E only
2. B and C only
3. C and D only
4. A and B only

Given below are two statements: In the light of the above statements, choose the most appropriate answer from the options given below :

1. Statement I is correct but statement II is incorrect
2. Statement I is incorrect but statement II is correct
3. Both statement I and statement II are correct
4. Both statement I and statement II are incorrect

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

1. 8 bp
2. 6 bp
3. 4 bp
4. 10 bp

What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism? Choose the correct answer from the options given below:

1. A and B only
2. D and E only
3. B and C only
4. A and E only

The "Ti plasmid" of Agrobacterium tumefaciens stands for

1. Tumour inhibiting plasmid
2. Tumor independent plasmid
3. Tumor inducing plasmid
4. Temperature independent plasmid

Which of the following statements is incorrect?

1. A bio-reactor provides optimal growth conditions for achieving the desired product
2. Most commonly used bio-reactors are of stirring type
3. Bio-reactors are used to produce small scale bacterial cultures
4. Bio-reactors have an agitator system, an oxygen delivery system and foam control system

The following diagram shows restriction sites in E. coli cloning vector pBR322. Find the role of 'X' and 'Y' genes : [figure]

1. The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid.
2. The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid.
3. The gene 'X' is for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics.
4. Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance.

In a chromosome, there is a specific DNA sequence, responsible for initiating replication. It is called as:

1. Recognition sequence
2. Cloning site
3. Restriction site
4. ori site

Identify the incorrect statement related to gel electrophoresis.

1. Separated DNA fragments can be directly seen under UV radiation
2. Separated DNA can be extracted from gel piece
3. Fragment of DNA moves toward anode
4. Sieving effect of agarose gel helps in separation of DNA fragments

Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:

1. Hind II, Hind II
2. Hind II, Alu I
3. Hind II, EcoR I
4. Hind II, BamHI

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR322 cloning vector.

1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI

Which of the following are correct about EcoRI? Choose the correct answer from the options given below:

1. B, C, E only
2. A, D, E only
3. A, C, D only
4. B, C, D only

Following are the steps involved in the process of PCR. Choose the correct sequence of steps of PCR from the options given below :

1. C A D E B
2. A B E D C
3. A C E D B
4. D B E C A

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

1. DNA
2. Histones
3. Polysaccharides
4. RNA

In gene gun method used to introduce alien DNA into host cells, microparticles of metal are used.

1. Zinc
2. Tungsten or gold
3. Silver
4. Copper

Upon exposure to UV radiation, DNA stained with ethidium bromide will show

1. Bright blue colour
2. Bright yellow colour
3. Bright orange colour
4. Bright red colour

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence. Choose the correct answer from the options given below :

1. C, A, B, D
2. C, B, D, A
3. B, D, A, C
4. B, C, D, A

Which of the following is not a cloning vector?

1. YAC
2. pBR322
3. Probe
4. BAC

Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R). In the light of the above statements, choose the correct answer from the options given below :

1. Both (A) and (R) are correct and (R) is the correct explanation of (A)
2. Both (A) and (R) are correct but (R) is not the correct explanation of (A)
3. (A) is correct but (R) is not correct
4. (A) is not correct but (R) is correct